Effect of filter extraction solvents on the measurement of the oxidative potential of airborne PM_2.5.

ABSTRACT: Solvent extraction of PM_2.5 samples collected on the filter is a preliminary step for assessing the PM_2.5 oxidative potential (OP) using cell-free assays, as the dithiothreitol (DTT) and the ascorbic acid (AA) assays. In this study, we evaluated the effect of the solvent choice by extracting ambient PM_2.5 samples with different solvents: methanol, as organic solvent, and two aqueous buffers, i.e., phosphate buffer (PB) and Gamble's solution (G), as a lung fluid surrogate solution. Both the measured volume-based OP_V^DTT and OP_V^AA responses varied for the different extraction methods, since methanol extraction generated the lowest values and phosphate buffer the highest. Although all the tested solvents produced inte

SUBMITTER: Pietrogrande MC 

PROVIDER: S-EPMC8222022 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature