A new tool for technical standardization of the Ki67 immunohistochemical assay.

ABSTRACT: Ki67, a nuclear proliferation-related protein, is heavily used in anatomic pathology but has not become a companion diagnostic or a standard-of-care biomarker due to analytic variability in both assay protocols and interpretation. The International Ki67 Working Group in breast cancer has published and has ongoing efforts in the standardization of the interpretation of Ki67, but they have not yet assessed technical issues of assay production representing multiple sources of variation, including antibody clones, antibody formats, staining platforms, and operators. The goal of this work is to address these issues with a new standardization tool. We have developed a cell line microarray system in which mixes of human Karpas 299 or Jurkat cells (Ki67⁺) with Sf9 (Spodoptera frugiperda

SUBMITTER: Aung TN 

PROVIDER: S-EPMC8222064 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature