Promoter G-quadruplexes and transcription factors cooperate to shape the cell type-specific transcriptome.

ABSTRACT: Cell identity is maintained by activation of cell-specific gene programs, regulated by epigenetic marks, transcription factors and chromatin organization. DNA G-quadruplex (G4)-folded regions in cells were reported to be associated with either increased or decreased transcriptional activity. By G4-ChIP-seq/RNA-seq analysis on liposarcoma cells we confirmed that G4s in promoters are invariably associated with high transcription levels in open chromatin. Comparing G4 presence, location and transcript levels in liposarcoma cells to available data on keratinocytes, we showed that the same promoter sequences of the same genes in the two cell lines had different G4-folding state: high transcript levels consistently associated with G4-folding. Transcription factors AP-1 and SP1, whose binding sit

SUBMITTER: Lago S 

PROVIDER: S-EPMC8222265 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature