Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography.

ABSTRACT: The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT) scanning approach is one method for SX sample delivery that minimizes sample consumption and minimizes physical damage to crystals during data collection. Settling of the crystals on the sample holder in random orientation is important for complete three dimensional data collection. To increase the random orientation of crystals on the sample holder, we developed a polyimide mesh-based sample holder with irregular crystal mounting holes for FT-SX. The polyimide mesh was fabricated using a picosecond laser. Each hole in the polyimi

SUBMITTER: Nam KH 

PROVIDER: S-EPMC8222285 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature