A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept.

ABSTRACT: Microfluidic-based tissues-on-chips (TOCs) have thus far been restricted to modelling simple epithelia as a single cell layer, but likely due to technical difficulties, no TOCs have been reported to include both an epithelial and a stromal component despite the biological importance of the stroma for the structure and function of human tissues. We present, for the first time, a novel approach to generate 3D multilayer tissue models in microfluidic platforms. As a proof of concept, we modelled skin, including a dermal and an epidermal compartment. To accomplish this, we developed a parallel flow method enabling the deposition of bilayer tissue in the upper chamber, which was subsequently maintained under dynamic nutrient flow conditions through the lower chamber, mimicking the function of a

SUBMITTER: Valencia L 

PROVIDER: S-EPMC8222336 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature