Single-cell transcriptomic analysis reveals disparate effector differentiation pathways in human T_reg compartment.

ABSTRACT: Human FOXP3⁺ regulatory T (T_reg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve T_reg cells from healthy individuals and patients with or without acute graft-versus-host disease (aGVHD) who undergo stem cell transplantation. These analyses, combined with functional assays, separate T_reg cells into naïve, activated, and effector stages, and resolve the HLA-DR^hi, LIMS1^hi, highly suppressive FOXP3^hi, and highly proliferative MKI67^hi effector subsets. Trajectory analysis assembles T_reg subsets into two differentiation paths (I/II) with distinctive phenotypi

SUBMITTER: Luo Y 

PROVIDER: S-EPMC8222404 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature