HCK maintains the self-renewal of leukaemia stem cells via CDK6 in AML.

ABSTRACT:

Background

Leukaemia stem cells (LSCs) are responsible for the initiation, maintenance, and recurrence of acute myeloid leukaemia (AML), an aggressive haematological malignancy associated with drug resistance and relapse. Identifying therapeutic LSC targets is critical to curing AML.

Methods

Bioinformatics databases were used to identify therapeutic LSC targets. The conditional knockout mice were used to analyse the role of HCK in leukaemogenesis or normal haematopoiesis. Colony-forming assays, cell counting, and flow cytometry were used to detect the viability and function of leukaemia cells. RT-PCR, western blotting, and RNA sequencing were used to detect mRNA and protein expression.

Result

HCK is expressed at higher levels in LSCs than in haematopoietic stem cells

SUBMITTER: Li Z 

PROVIDER: S-EPMC8223385 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature