Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures.

ABSTRACT: Cationic coatings can enhance the stability of synthetic DNA objects in low ionic strength environments such as physiological fluids. Here, we used single-particle cryo-electron microscopy (cryo-EM), pseudoatomic model fitting, and single-molecule mass photometry to study oligolysine and polyethylene glycol (PEG)-oligolysine-coated multilayer DNA origami objects. The coatings preserve coarse structural features well on a resolution of multiple nanometers but can also induce deformations such as twisting and bending. Higher-density coatings also led to internal structural deformations in the DNA origami test objects, in which a designed honeycomb-type helical lattice was deformed into a more square-lattice-like pattern. Under physiological ionic strength, where the uncoated objects disassem

SUBMITTER: Bertosin E 

PROVIDER: S-EPMC8223477 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature