MinION nanopore sequencing and assembly of a complete human papillomavirus genome.

ABSTRACT:

Background

The MinION sequencer belongs to the third generation of sequencing technology that allows for the generation of ultra-long reads, representing a potentially more effective approach to characterize entire viral genome sequences than other time-consuming and low-throughput methodologies.

Methods

We report the use of the MinION nanopore sequencer to sequence the full-length genome of human papillomavirus (HPV)-ICB2 (7441 bp), which was previously characterized in our laboratory. Three independent MinION libraries were prepared and sequenced using either three consecutive 12 -h runs (Protocol A) or a single run of 48 h starting from a pool of three barcoded DNA libraries (Protocol B). A fully automated bioinformatics pipeline was developed for the reconstruction of th

SUBMITTER: Brancaccio RN 

PROVIDER: S-EPMC8223502 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature