Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging.

ABSTRACT: Live imaging of Drosophila melanogaster ovaries has been instrumental in understanding a variety of basic cellular processes during development, including ribonucleoprotein particle movement, mRNA localization, organelle movement, and cytoskeletal dynamics. There are several methods for live imaging that have been developed. Due to the fact that each method involves dissecting individual ovarioles placed in media or halocarbon oil, cellular damage due to hypoxia and/or physical manipulation will inevitably occur over time. One downstream effect of hypoxia is to increase oxidative damage in the cells. The purpose of this protocol is to use live imaging to visualize the effects of oxidative damage on the localization and dynamics of subcellular structures in Drosophila ovaries after inductio

SUBMITTER: Sheard KM 

PROVIDER: S-EPMC8223520 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature