Karyotype engineering by chromosome fusion leads to reproductive isolation in yeast.

ABSTRACT: Extant species have wildly different numbers of chromosomes, even among taxa with relatively similar genome sizes (for example, insects)^1,2. This is likely to reflect accidents of genome history, such as telomere-telomere fusions and genome duplication events^3-5. Humans have 23 pairs of chromosomes, whereas other apes have 24. One human chromosome is a fusion product of the ancestral state⁶. This raises the question: how well can species tolerate a change in chromosome numbers without substantial changes to genome content? Many tools are used in chromosome engineering in Saccharomyces cerevisiae^7-10, but CRISPR-Cas9-mediated genome editing facilitates the most aggressive engineering strategies. Here we successfully fused yeast chromosomes using C

SUBMITTER: Luo J 

PROVIDER: S-EPMC8223741 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature