Reconstructing a Wild-Type Pseudomonas aeruginosa Reference Strain PAO1.

ABSTRACT: The P. aeruginosa reference strain PAO1 has been used to delineate much of the physiology, metabolism, and fundamental biology of the species. The wild-type parent of PAO1 was lost, and PAO1 carries a regulatory mutation introduced for positive genetic selection that affects antibiotic resistance, virulence, quorum sensing, and other traits. The mutation is a loss-of-function change in an oxidoreductase gene (mexS), which constitutively activates a stress response controlled by a positive regulator (MexT). Fitness defects associated with the constitutive response have led to the inadvertent selection of mexT-minus suppressor mutations, creating genetic heterogeneity in PAO1 sublines studied in different laboratories. To help circumvent complications due to the mexS-min

SUBMITTER: Lee S 

PROVIDER: S-EPMC8223932 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature