Pepscan Approach for the Identification of Protein-Protein Interfaces: Lessons from Experiment.

ABSTRACT: PEPscan is an old approach that has recently gained renewed interest for the identification of interfering peptides (IPs), i.e., peptides able to interfere with protein-protein interactions (PPIs). Its principle is to slice a protein sequence as a series of short overlapping peptides that are synthesized on a peptide array and tested for their ability to bind a partner, with positive spots corresponding to candidate IPs. PEPscan has been applied with a rather large success in various contexts, but the structural determinants underlying this success remain obscure. Here, we analyze the results of 14 PEPscan experiments, and confront the in vitro results with the available structural information. PEPscan identifies candidate IPs in limited numbers that in all cases correspond to solvent-acce

SUBMITTER: Rebollo A 

PROVIDER: S-EPMC8224071 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature