SARS-CoV-2 detection and genomic sequencing from hospital surface samples collected at UC Davis.

ABSTRACT:

Rationale

There is little doubt that aerosols play a major role in the transmission of SARS-CoV-2. The significance of the presence and infectivity of this virus on environmental surfaces, especially in a hospital setting, remains less clear.

Objectives

We aimed to analyze surface swabs for SARS-CoV-2 RNA and infectivity, and to determine their suitability for sequence analysis.

Methods

Samples were collected during two waves of COVID-19 at the University of California, Davis Medical Center, in COVID-19 patient serving and staff congregation areas. qRT-PCR positive samples were investigated in Vero cell cultures for cytopathic effects and phylogenetically assessed by whole genome sequencing.

Measurements and main results

Improved cleaning and patient management

SUBMITTER: Coil DA 

PROVIDER: S-EPMC8224861 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature