Exposure to traffic-related air pollution and bacterial diversity in the lower respiratory tract of children.

ABSTRACT:

Background

Exposure to particulate matter has been shown to increase the adhesion of bacteria to human airway epithelial cells. However, the impact of traffic-related air pollution (TRAP) on the respiratory microbiome is unknown.

Methods

Forty children were recruited through the Cincinnati Childhood Allergy and Air Pollution Study, a longitudinal cohort followed from birth through early adolescence. Saliva and induced sputum were collected at age 14 years. Exposure to TRAP was characterized from birth through the time of sample collection using a previously validated land-use regression model. Sequencing of the bacterial 16S and ITS fungal rRNA genes was performed on sputum and saliva samples. The relative abundance of bacterial taxa and diversity indices were compared in ch

SUBMITTER: Niemeier-Walsh C 

PROVIDER: S-EPMC8224880 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature