MHCEpitopeEnergy, a Flexible Rosetta-Based Biotherapeutic Deimmunization Platform.

ABSTRACT: As non-"self" macromolecules, biotherapeutics can trigger an immune response that can reduce drug efficacy, require patients to be taken off therapy, or even cause life-threatening reactions. To enable the flexible and facile design of protein biotherapeutics while reducing the prevalence of T-cell epitopes that drive immune recognition, we have integrated into the Rosetta protein design suite a new scoring term that allows design protocols to account for predicted or experimentally identified epitopes in the optimized objective function. This flexible scoring term can be used in any Rosetta design trajectory, can be targeted to specific regions of a protein, and can be readily extended to work with a variety of epitope predictors. By performing extensive design runs with varied design par

SUBMITTER: Yachnin BJ 

PROVIDER: S-EPMC8225355 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature