Differences in in vitro microglial accumulation of the energy metabolism tracers [¹⁸F]FDG and [¹⁸F]BCPP-EF during LPS- and IL4 stimulation.

ABSTRACT: The positron emission tomography probes 2-deoxy-2-[¹⁸F]fluoro-D-glucose ([¹⁸F]FDG) and 2-tert-butyl-4-chloro-5-{6-[2-(2-[¹⁸F]fluoroethoxy)-ethoxy]-pyridin-3-ylmethoxy}-2H-pyridazin-3-one ([¹⁸F]BCPP-EF) are designed to evaluate glycolysis and oxidative phosphorylation, respectively, and are both used to estimate neuronal activity. However, previous studies have shown a discrepancy in these probes' accumulation in the compromised region, possibly due to the presence of activated microglia acting like deleterious or neuroprotective phenotypes. Hence, we evaluated lipopolysaccharide (LPS)- and interleukin 4 (IL4)-stimulated microglial uptake of [¹⁴C]2DG and [¹⁸F]BCPP-EF to give a new insight into the hypothesis that different up

SUBMITTER: Suzuki C 

PROVIDER: S-EPMC8225620 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature