Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO₂ treatment.

ABSTRACT: Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO₂ availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference f

SUBMITTER: Azarfar G 

PROVIDER: S-EPMC8225881 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature