Differential Exoproteome and Biochemical Characterisation of Neoparamoeba perurans.

ABSTRACT: Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amalgamated database of bacterial, N. perurans and Amoebozoa proteins. LC-MS/MS identified 33 differentially expressed proteins, the majority of which were upregulated in the attenuated exoproteome. Proteins of putative interest found in both exoproteomes were maltoporin, ferrichrome-iron receptor, and putative ferric enterobactin receptor. Protease activity remained signif

SUBMITTER: Ni Dhufaigh K 

PROVIDER: S-EPMC8226569 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature