Degradation of the Indospicine Toxin from Indigofera spicata by a Mixed Population of Rumen Bacteria.

ABSTRACT: The leguminous plant species, Indigofera linnaei and Indigofera spicata are distributed throughout the rangeland regions of Australia and the compound indospicine (L-2-amino-6-amidinohexanoic acid) found in these palatable forage plants acts as a hepatotoxin and can accumulate in the meat of ruminant livestock and wild camels. In this study, bovine rumen fluid was cultivated in an in vitro fermentation system provided with Indigofera spicata plant material and the ability of the resulting mixed microbial populations to degrade indospicine was determined using UPLC-MS/MS over a 14 day time period. The microbial populations of the fermentation system were determined using 16S rRNA gene amplicon sequencing and showed distinct, time-related changes occurring as the rumen-d

SUBMITTER: Gilbert RA 

PROVIDER: S-EPMC8226729 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature