Identification of Homologous Polyprenols from Thermophilic Bacteria.

ABSTRACT: Sixteen strains of five genera of thermophilic bacteria, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus, were cultivated at a temperature from 42 to 70 °C. Twelve strains were obtained from the Czech Collection of Microorganisms, while four were directly isolated and identified by 16S rRNA gene sequencing from the hot springs of the world-famous Carlsbad spa (Czech Republic). Polyprenol homologs from C40 to C65 as well as free undecaprenol (C55), undecaprenyl phosphate, and undecaprenyl diphosphate were identified by shotgun analysis and RP-HPLC/MS-ESI⁺ (reverse phase high-performance liquid chromatography-high-resolution positive electrospray ionization mass spectrometry). The limit of detection (50 pM) was determined for individual h

SUBMITTER: Gharwalova L 

PROVIDER: S-EPMC8226974 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature