Nano- and Micro-Porous Chitosan Membranes for Human Epidermal Stratification and Differentiation.

ABSTRACT: The creation of partial or complete human epidermis represents a critical aspect and the major challenge of skin tissue engineering. This work was aimed at investigating the effect of nano- and micro-structured CHT membranes on human keratinocyte stratification and differentiation. To this end, nanoporous and microporous membranes of chitosan (CHT) were prepared by phase inversion technique tailoring the operational parameters in order to obtain nano- and micro-structured flat membranes with specific surface properties. Microporous structures with different mean pore diameters were created by adding and dissolving, in the polymeric solution, polyethylene glycol (PEG Mw 10,000 Da) as porogen, with a different CHT/PEG ratio. The developed membranes were characterized and assessed for epiderm

SUBMITTER: Salerno S 

PROVIDER: S-EPMC8227018 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature