The Full-Length Transcriptome Sequencing and Identification of Na⁺/H⁺ Antiporter Genes in Halophyte Nitraria tangutorum Bobrov.

ABSTRACT: Nitraria tangutorum Bobrov is a halophyte that is resistant to salt and alkali and is widely distributed in northwestern China. However, its genome has not been sequenced, thereby limiting studies on this particular species. For species without a reference genome, the full-length transcriptome is a convenient and rapid way to obtain reference gene information. To better study N. tangutorum, we used PacBio single-molecule real-time technology to perform full-length transcriptome analysis of this halophyte. In this study, a total of 21.83 Gb of data were obtained, and 198,300 transcripts, 51,875 SSRs (simple sequence repeats), 55,574 CDS (coding sequence), and 74,913 lncRNAs (long non-coding RNA) were identified. In addition, using this full-length transcriptome, we identified

SUBMITTER: Zhu L 

PROVIDER: S-EPMC8227117 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature