Heparan Sulfate Proteoglycans as Attachment Factor for SARS-CoV-2.

ABSTRACT: Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is causing an unprecedented global pandemic demanding the urgent development of therapeutic strategies. Microarray binding experiments, using an extensive heparan sulfate (HS) oligosaccharide library, showed that the receptor binding domain (RBD) of the spike of SARS-CoV-2 can bind HS in a length- and sequence-dependent manner. A hexasaccharide composed of IdoA2S-GlcNS6S repeating units was identified as the minimal binding epitope. Surface plasmon resonance showed the SARS-CoV-2 spike protein binds with a much higher affinity to heparin (K _D = 55 nM) compared to the RBD (K _D = 1 μM) alone. It was also found that heparin does not interfere in angiotensin-converting enzyme 2 (ACE2) bindi

SUBMITTER: Liu L 

PROVIDER: S-EPMC8227597 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature