A Senescence-Like Cellular Response Inhibits Bovine Ephemeral Fever Virus Proliferation.

ABSTRACT: During industrial-scale production of viruses for vaccine manufacturing, anti-viral response of host cells can dampen maximal viral antigen yield. In addition to interferon responses, many other cellular responses, such as the AMPK signaling pathway or senescence-like response may inhibit or slow down virus amplification in the cell culture system. In this study, we first performed a Gene Set Enrichment Analysis of the whole-genome mRNA transcriptome and found a senescence-like cellular response in BHK-21 cells when infected with bovine ephemeral fever virus (BEFV). To demonstrate that this senescence-like state may reduce virus growth, BHK-21 subclones showing varying degrees of a senescence-like state were infected with BEFV. The results showed that the BHK-21 subclones showing high sene

SUBMITTER: Zeng YJ 

PROVIDER: S-EPMC8227762 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature