Different Roles of p62 (SQSTM1) Isoforms in Keratin-Related Protein Aggregation.

ABSTRACT: p62/Sequestosome-1 (p62) is a multifunctional adaptor protein and is also a constant component of disease-associated protein aggregates, including Mallory-Denk bodies (MDBs), in steatohepatitis and hepatocellular carcinoma. We investigated the interaction of the two human p62 isoforms, p62-H1 (full-length isoform) and p62-H2 (partly devoid of PB1 domain), with keratins 8 and 18, the major components of MDBs. In human liver, p62-H2 is expressed two-fold higher compared to p62-H1 at the mRNA level and is present in slightly but not significantly higher concentrations at the protein level. Co-transfection studies in CHO-K1 cells, PLC/PRF/5 cells as well as p62^- total-knockout and wild-type mouse fibroblasts revealed marked differences in the cytoplasmic distribution and aggregation

SUBMITTER: Somlapura M 

PROVIDER: S-EPMC8227998 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature