Performance of the LIAISON^® SARS-CoV-2 Antigen Assay vs. SARS-CoV-2-RT-PCR.

ABSTRACT: We aimed to evaluate the LIAISON^® SARS-CoV-2 antigen assay (DiaSorin), comparing its performance to real-time polymerase chain reaction (RT-PCR) for the detection of SARS-CoV-2 RNA. 182 (110 PCR-positive and 72 PCR-negative) nasopharyngeal swab samples were taken for the detection of SARS-CoV-2. RT-PCR and antigen assay were performed using the same material. The sensitivity and specificity of the antigen assay were calculated for different cut-offs, with RT-PCR serving as the reference method. Stored clinical samples that were positive for other respiratory viruses were tested to evaluate cross-reactivity. One third (33/110, 30%) were falsely classified as negative, while no false positives were found using the 200 TCID₅₀/mL cut-off for the SARS-CoV-2 antigen as prop

SUBMITTER: Fiedler M 

PROVIDER: S-EPMC8228578 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature