Cascade Strand Displacement and Bipedal Walking Based DNA Logic System for miRNA Diagnostics.

ABSTRACT: DNA logic gated operations empower the highly efficient analysis of multiplex nucleic acid inputs, which have attracted extensive attention. However, the integration of DNA logic gates with abundant computational functions and signal amplification for biomedical diagnosis is far from being fully achieved. Herein, we develop a bipedal DNA walker based amplified electrochemical method for miRNA detection, which is then used as the basic unit for the construction of various logic circuits, enabling the analysis of multiplex miRNAs. In the bipedal walking process, target triggered strand displacement polymerization is able to produce a large number of strands for the fabrication of three-way junction-structured bipedal walkers. The following catalytic hairpin assembly ensures the walking event

SUBMITTER: Miao P 

PROVIDER: S-EPMC8228592 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature