Guide to Semi-Quantitative Non-Targeted Screening Using LC/ESI/HRMS.

ABSTRACT: Non-targeted screening (NTS) with reversed phase liquid chromatography electrospray ionization high resolution mass spectrometry (LC/ESI/HRMS) is increasingly employed as an alternative to targeted analysis; however, it is not possible to quantify all compounds found in a sample with analytical standards. As an alternative, semi-quantification strategies are, or at least should be, used to estimate the concentrations of the unknown compounds before final decision making. All steps in the analytical chain, from sample preparation to ionization conditions and data processing can influence the signals obtained, and thus the estimated concentrations. Therefore, each step needs to be considered carefully. Generally, less is more when it comes to choosing sample preparation as well as chromatogr

SUBMITTER: Malm L 

PROVIDER: S-EPMC8228683 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature