Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements.

ABSTRACT: The mesoporous nature of silica nanoparticles provides a novel platform for the development of ultrabright fluorescent particles, which have organic molecular fluorescent dyes physically encapsulated inside the silica pores. The close proximity of the dye molecules, which is possible without fluorescence quenching, gives an advantage of building sensors using FRET coupling between the encapsulated dye molecules. Here we present the use of this approach to demonstrate the assembly of ultrabright fluorescent ratiometric sensors capable of simultaneous acidity (pH) and temperature measurements. FRET pairs of the temperature-responsive, pH-sensitive and reference dyes are physically encapsulated inside the silica matrix of ~50 nm particles. We demonstrate that the particles can be used to meas

SUBMITTER: Peerzade SAMA 

PROVIDER: S-EPMC8228773 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature