Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids.

ABSTRACT: Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. In addition, we discuss approaches for EV imaging and identify the potential benefits and disadva

SUBMITTER: Malenica M 

PROVIDER: S-EPMC8228884 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature