Dynamic Physiological Culture of Ex Vivo Human Tissue: A Systematic Review.

ABSTRACT: Conventional static culture fails to replicate the physiological conditions that exist in vivo. Recent advances in biomedical engineering have resulted in the creation of novel dynamic culturing systems that permit the recapitulation of normal physiological processes ex vivo. Whilst the physiological benefit for its use in the culture of two-dimensional cellular monolayer has been validated, its role in the context of primary human tissue culture has yet to be determined. This systematic review identified 22 articles that combined dynamic physiological culture techniques with primary human tissue culture. The most frequent method described (55%) utilised dynamic perfusion culture. A diverse range of primary human tissue was successfully cultured. The median duration of successful ex vivo c

SUBMITTER: Hughes DL 

PROVIDER: S-EPMC8229413 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature