Semi-Automated Cell Panning for Efficient Isolation of FGFR3-Targeting Antibody.

ABSTRACT: Phage display technology is a widely used practical tool for isolating binding molecules against the desired targets in phage libraries. In the case of targeting the membrane protein with its natural conformation, conventional bio-panning has limitations on the efficient screening of the functionally relevant antibodies. To enrich the single-chain variable fragment (scFv) pools for recognizing the natural conformation of the membrane targets, the conventional bio-panning and screening process was modified to include the semi-automated cell panning protocol. Using FGFR3-overexpressing patient-derived cancer cells, biotin-X-DHPE was introduced and coupled to Streptavidin-coated magnetic beads for use in the solution-phage bio-panning procedure. The resulting clones of scFv were compared to t

SUBMITTER: Min B 

PROVIDER: S-EPMC8229736 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature