Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model.

ABSTRACT: Cryptosporidiosis is a major human health concern globally. Despite well-established methods, misdiagnosis remains common. Our understanding of the cryptosporidiosis biochemical mechanism remains limited, compounding the difficulty of clinical diagnosis. Here, we used a systems biology approach to investigate the underlying biochemical interactions in C57BL/6J mice infected with Cryptosporidium parvum. Faecal samples were collected daily following infection. Blood, liver tissues and luminal contents were collected 10 days post infection. High-resolution liquid chromatography and low-resolution gas chromatography coupled with mass spectrometry were used to analyse the proteomes and metabolomes of these samples. Faeces and luminal contents were additionally subjected to 16S rRNA gene

SUBMITTER: Karpe AV 

PROVIDER: S-EPMC8230837 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature