A novel proline-rich M-superfamily conotoxin that can simultaneously affect sodium, potassium and calcium currents.

ABSTRACT:

Background

Conotoxins have become a research hotspot in the neuropharmacology field for their high activity and specificity in targeting ion channels and neurotransmitter receptors. There have been reports of a conotoxin acting on two ion channels, but rare reports of a conotoxin acting on three ion channels.

Methods

Vr3a, a proline-rich M-superfamily conotoxin from a worm-hunting Conus varius, was obtained by solid-phase synthesis and identified by mass spectrometry. The effects of synthesized Vr3a on sodium, potassium and calcium currents were tested on rat DRG cells by patch clamp experiments. The further effects of Vr3a on human Ca_v1.2 and Ca_v2.2 currents were tested on HEK293 cells.

Results

About 10 μM Vr3a has no effects on the peak

SUBMITTER: Yang M 

PROVIDER: S-EPMC8230863 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature