Quality Assessment and Validation of High-Throughput Sequencing for Grapevine Virus Diagnostics.

ABSTRACT: Development of High-Throughput Sequencing (HTS), also known as next generation sequencing, revolutionized diagnostic research of plant viruses. HTS outperforms bioassays and molecular diagnostic assays that are used to screen domestic and quarantine grapevine materials in data throughput, cost, scalability, and detection of novel and highly variant virus species. However, before HTS-based assays can be routinely used for plant virus diagnostics, performance specifications need to be developed and assessed. In this study, we selected 18 virus-infected grapevines as a test panel for measuring performance characteristics of an HTS-based diagnostic assay. Total nucleic acid (TNA) was extracted from petioles and dormant canes of individual samples and constructed libraries were run on Illumina

SUBMITTER: Soltani N 

PROVIDER: S-EPMC8231206 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature