Methylation of CYP1A1 and VKORC1 promoter associated with stable dosage of warfarin in Chinese patients.

ABSTRACT:

Objective

To investigate the association between DNA methylation and the stable warfarin dose through genome-wide DNA methylation analysis and pyrosequencing assay.

Method

This study included 161 patients and genome-wide DNA methylation analysis was used to screen potential warfarin dose-associated CpGs through Illumina Infinium HumanMethylation 450 K BeadChip; then, the pyrosequencing assay was used to further validate the association between the stable warfarin dose and alterations in the methylation of the screened CpGs. GenomeStudio Software and R were used to analyze the differentially methylated CpGs.

Results

The methylation levels of CpGs surrounding the xenobiotic response element (XRE) within the CYP1A1 promoter, differed significantly between the different d

SUBMITTER: He S 

PROVIDER: S-EPMC8231338 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature