A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts.

ABSTRACT: Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (E_W) from phage ΦX174 and the screened mutant protein E (E_M) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein E_M was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD₆₀₀ = 2.0, which was higher c

SUBMITTER: Ma Y 

PROVIDER: S-EPMC8231862 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature