Development of a Modular Vaccine Platform for Multimeric Antigen Display Using an Orthobunyavirus Model.

ABSTRACT: Emerging infectious diseases represent an increasing threat to human and animal health. Therefore, safe and effective vaccines that could be available within a short time frame after an outbreak are required for adequate prevention and control. Here, we developed a robust and versatile self-assembling multimeric protein scaffold particle (MPSP) vaccine platform using lumazine synthase (LS) from Aquifex aeolicus. This scaffold allowed the presentation of peptide epitopes by genetic fusion as well as the presentation of large antigens by bacterial superglue-based conjugation to the pre-assembled particle. Using the orthobunyavirus model Schmallenberg virus (SBV) we designed MPSPs presenting major immunogens of SBV and assessed their efficacy in a mouse model as well as in cattle, a ta

SUBMITTER: Aebischer A 

PROVIDER: S-EPMC8232151 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature