Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos.

ABSTRACT: Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25-25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation

SUBMITTER: Canovas S 

PROVIDER: S-EPMC8232708 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature