Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans.

ABSTRACT: High homocysteine (Hcy) levels, mainly caused by vitamin B12 deficiency, have been reported to induce amyloid-β (Aβ) formation and tau hyperphosphorylation in mouse models of Alzheimer's disease. However, the relationship between B12 deficiency and Aβ aggregation is poorly understood, as is the associated mechanism. In the current study, we used the transgenic C. elegans strain GMC101, which expresses human Aβ1-42 peptides in muscle cells, to investigate the effects of B12 deficiency on Aβ aggregation-associated paralysis. C. elegans GMC101 was grown on nematode growth medium with or without B12 supplementation or with 2-O-α-D-glucopyranosyl-L-ascorbic acid (AsA-2G) supplementation. The worms were age-synchronized by hypochlorite bleaching and incubated at 20 °C. After the worms reached th

SUBMITTER: Andra A 

PROVIDER: S-EPMC8232795 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature