SIRT1-dependent restoration of NAD+ homeostasis after increased extracellular NAD+ exposure.

ABSTRACT: In the last several years, NAD+ supplementation has emerged as an innovative and safe therapeutic strategy for a wide spectrum of disorders, including diabetes and neuropathy. However, critical questions remain as to how NAD+ and its precursors are taken up by cells, as well as the effects of long-lasting intracellular NAD+ (iNAD+) increases. Here, we investigated the kinetics of iNAD+ levels in different cell types challenged with prolonged exposure to extracellular NAD+ (eNAD+). Surprisingly, we found that after the initial increase, iNAD+ contents decreased back to control levels (iNAD+ resetting). Focusing our attention on HeLa cells, we found that oxygen and ATP consumption occurred with similar temporal kinetics after eNAD+ exposure. Using [³H]NAD+ and [¹⁴C]NAD+

SUBMITTER: Buonvicino D 

PROVIDER: S-EPMC8233143 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature