Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition.

ABSTRACT: KRAS, an oncogene, is frequently activated by mutations in many cancers. Kras-driven adenocarcinoma development in the lung, pancreas, and biliary tract has been extensively studied using gene targeting in mice. By taking the organoid- and allograft-based genetic approach to these organs, essentially the same results as in vivo models were obtained in terms of tumor development. To verify the applicability of this approach to other organs, we investigated whether the combination of Kras activation and Pten inactivation, which gives rise to endometrial tumors in mice, could transform murine endometrial organoids in the subcutis of immunodeficient mice. We found that in Kras^G12D-expressing endometrial organoids, Pten knockdown did not confer tumorigenicity, but Cdkn2a knockdown or

SUBMITTER: Maru Y 

PROVIDER: S-EPMC8233399 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature