Analysing Parallel Strategies to Alter the Host Specificity of Bacteriophage T7.

ABSTRACT: The recognition and binding of host bacteria by bacteriophages is most often enabled by a highly specific receptor-ligand type of interaction, with the receptor-binding proteins (RBPs) of phages being the primary determinants of host specificity. Specifically modifying the RBPs could alter or extend the host range of phages otherwise exhibiting desired phenotypic properties. This study employed two different strategies to reprogram T7 phages ordinarily infecting commensal K12 Escherichia coli strains to infect pathogen-associated K1-capsule-expressing strains. The strategies were based on either plasmid-based homologous recombination or bacteriophage recombineering using electroporated DNA (BRED). Our work pursued the construction of two genetic designs: one replacing the gp17 gene of T7,

SUBMITTER: Avramucz A 

PROVIDER: S-EPMC8234382 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature