T-box transcription factor 21 is expressed in terminal Schwann cells at the neuromuscular junction.

ABSTRACT:

Introduction/aims

Terminal Schwann cells (tSCs) are nonmyelinating Schwann cells present at the neuromuscular junction (NMJ) with multiple integral roles throughout their lifespan. There is no known gene differentiating tSCs from myelinating Schwann cells, making their isolation and investigation challenging. In this work we investigated genes expressed within tSCs.

Methods

A novel dissection technique was utilized to isolate the tSC-containing NMJ band from the sternomastoid muscles of S100-GFP mice. RNA was isolated from samples containing: (a) NMJ bands (tSCs with nerve and muscle), (b) nerve, and (c) muscle, and microarray genetic expression analysis was conducted. Data were validated by quantitative real-time polymerase chain reaction (qRT-PCR) and immunofluorescent sta

SUBMITTER: Jablonka-Shariff A 

PROVIDER: S-EPMC8235608 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature