Development of a Bio-Layer Interferometry-Based Protease Assay Using HIV-1 Protease as a Model.

ABSTRACT: Proteolytic enzymes have great significance in medicine and the pharmaceutical industry and are applied in multiple fields of life sciences. Therefore, cost-efficient, reliable and sensitive real-time monitoring methods are highly desirable to measure protease activity. In this paper, we describe the development of a new experimental approach for investigation of proteolytic enzymes. The method was designed by the combination of recombinant fusion protein substrates and bio-layer interferometry (BLI). The protease (PR) of human immunodeficiency virus type 1 (HIV-1) was applied as model enzyme to set up and test the method. The principle of the assay is that the recombinant protein substrates immobilized to the surface of biosensor are specifically cleaved by the PR, and the substrate proce

SUBMITTER: Miczi M 

PROVIDER: S-EPMC8235736 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature