Activated neutrophils polarize protumorigenic interleukin-17A-producing T helper subsets through TNF-α-B7-H2-dependent pathway in human gastric cancer.

ABSTRACT:

Rationale

Neutrophils constitute massive cellular constituents in inflammatory human gastric cancer (GC) tissues, but their roles in pathogenesis of inflammatory T helper (Th) subsets are still unknown.

Methods

Flow cytometry analysis and immunohistochemistry were used to analyze the responses and phenotypes of neutrophils in different samples from 51 patients with GC. Kaplan-Meier plots and Multivariate analysis for the survival of patients were used by log-rank tests and Cox proportional hazards models. Neutrophils and CD4⁺ T cells were purified and cultured for ex vivo, in vitro and in vivo regulation and function assays.

Results

GC patients exhibited increased tumoral neutrophil infiltration with GC progression and poor patient prognosis. Intratumoral n

SUBMITTER: Shan ZG 

PROVIDER: S-EPMC8236123 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature