Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

ABSTRACT:

Background

Phenotypic and functional heterogeneity of macrophages is known to be the main reason for their ability to regulate inflammation and promote tumorigenesis. Mesenchymal stem cells (MSCs) are one of the principal cells commonly found in the tumor stromal niche, with capability of macrophage phenotypic switching. The objective of this study was to evaluate the role of C-X-C motif chemokine ligand 12 (CXCL12) produced by marrow-derived MSCs in the phenotypic and functional pattern of bone marrow-derived macrophages (BMDMs).

Methods

First, the CRISPR/Cas9 system was used for the CXCL12 gene knock-out in MSCs. Then, coculture systems were used to investigate the role of MSCs^CXCL12-/- and MSCs^CXCL12+/+ in determination of macrophage phenotype. To fu

SUBMITTER: Babazadeh S 

PROVIDER: S-EPMC8236206 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature