Preconceptional smoking alters spermatozoal miRNAs of murine fathers and affects offspring's body weight.

ABSTRACT:

Background

Active smoking has been reported among 7% of teenagers worldwide, with ages ranging from 13 to 15 years. An epidemiological study suggested that preconceptional paternal smoking is associated with adolescent obesity in boys. We developed a murine adolescent smoking model before conception to investigate the paternal molecular causes of changes in offspring's phenotype.

Method

Male and female C57BL/6J mice were exposed to increasing doses of mainstream cigarette smoke (CS) from onset of puberty for 6 weeks and mated with room air (RA) controls.

Results

Thirteen miRNAs were upregulated and 32 downregulated in the spermatozoa of CS-exposed fathers, while there were no significant differences in the count and morphological integrity of spermatozoa, as well as t

SUBMITTER: Hammer B 

PROVIDER: S-EPMC8236406 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature