Efficient Secretion and Recombinant Production of a Lactobacillal α-amylase in <i>Lactiplantibacillus plantarum</i> WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides.

ABSTRACT: Lactic acid bacteria (LAB) have been used as starter cultures and producers of enzymes, antimicrobial peptides or metabolites that contribute to the flavor, texture and safety of food products. Lactiplantibacillus plantarum, one of the best-studied LAB, is considered as safe and effective cell factory for food applications. In this study, our aim was to use L. plantarum as the producer for high levels of a food-grade lactobacillal α-amylase, which has potential applications in food, fermentation and feed industries. The native form of an α-amylase (AmyL) from L. plantarum S21, an amylolytic LAB isolated from Thai fermented rice noodles, was expressed in L. plantarum WCFS1 using the pSIP expression system. The secretion of the α-amylase was driven by the native s

SUBMITTER: Tran AM 

PROVIDER: S-EPMC8236982 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature